Product name: Polymorphprep™
Delivery conditions: Ambient temperature
Delivery time in working days
• Europe: 1-2 days
• USA and Canada: 2-3 days
• Rest of the World: 3-7 days
- Polymorphprep™ should be stored at room temperature (20°C).
- It is stable for 3 years as long as the solution is kept sterile and protected from light.
- Prolonged exposure to direct sunlight causes the release of iodine from the diatrizoate sodium molecule (this effect is negligible when working with the solution on a day-to-day basis).
Applications: Isolation of human polymorphonuclear cells
The solution contains Sodium Diatrizoate and Dextran 500 in the following concentrations:
• Sodium diatrizoate 13.8% (w/v)
• Dextran 500 8.0 (w/v)
How Polymorphprep™ works
Polymorphonuclear leukocytes (PMNs) have a lower buoyant density than erythrocytes (1.09-1.11 g/ml) but a higher buoyant density than mononuclear cells (greater than 1.085 g/ml). Therefore, it is difficult to isolate polymorphonuclear granulocytes using a similar density barrier to that used for mononuclear cells. Various procedures have been developed to overcome these problems.
The high osmolality of Polymorphprep™ causes red blood cells to lose water and shrink, thereby increasing their effective buoyant densities. This allows the dextran-aggregated erythrocytes to rapidly sediment through the dense medium. As the cells settle further into the medium, the osmotic gradient between the medium and the erythrocytes decreases. This means that the loss of water from the red blood cells is greatest at the top of the Polymorphprep™ and decreases progressively as they settle more. A diatrizoate gradient forms within the density barrier. PMNs form a band within this density gradient while mononuclear cells remain at the sample/medium interface.
Polymorphprep™ vs. Ficoll-Paque
- Ficoll-Paque (GE Health Care) is not tested for endotoxins at a basic level and at a premium price
- Unlike Polymorphprep, Ficoll-Paque is not manufactured under EU cGMP compliance.
Alternatives: Polymorphprep™ can be a good substitute for Ficoll-Paque