Skip to content
L’Union des Fédérations d’Apiculture de Wallonie et Bruxelles

L’Union des Fédérations d’Apiculture de Wallonie et Bruxelles

UFAWB, honey ELISA residu testing, Quantichome

Menu
  • Home
  • Product
  • paraffin Liquid
  • Distributors
  • Blog
Menu

Polymorphprep

Posted on december 26, 2022 by Beau

Product name: Polymorphprep™

Delivery conditions: Ambient temperature

Delivery time in working days

• Europe: 1-2 days
• USA and Canada: 2-3 days
• Rest of the World: 3-7 days

Storage condition

  • Polymorphprep™ should be stored at room temperature (20°C).
  • It is stable for 3 years as long as the solution is kept sterile and protected from light.
  • Prolonged exposure to direct sunlight causes the release of iodine from the diatrizoate sodium molecule (this effect is negligible when working with the solution on a day-to-day basis).

Brand: Axis-shield

Applications: Isolation of human polymorphonuclear cells

Composition

The solution contains Sodium Diatrizoate and Dextran 500 in the following concentrations:

• Sodium diatrizoate 13.8% (w/v)
• Dextran 500 8.0 (w/v)

How Polymorphprep™ works

Polymorphonuclear leukocytes (PMNs) have a lower buoyant density than erythrocytes (1.09-1.11 g/ml) but a higher buoyant density than mononuclear cells (greater than 1.085 g/ml). Therefore, it is difficult to isolate polymorphonuclear granulocytes using a similar density barrier to that used for mononuclear cells. Various procedures have been developed to overcome these problems.

The high osmolality of Polymorphprep™ causes red blood cells to lose water and shrink, thereby increasing their effective buoyant densities. This allows the dextran-aggregated erythrocytes to rapidly sediment through the dense medium. As the cells settle further into the medium, the osmotic gradient between the medium and the erythrocytes decreases. This means that the loss of water from the red blood cells is greatest at the top of the Polymorphprep™ and decreases progressively as they settle more. A diatrizoate gradient forms within the density barrier. PMNs form a band within this density gradient while mononuclear cells remain at the sample/medium interface.

Advantage

Polymorphprep™ vs. Ficoll-Paque

  • Ficoll-Paque (GE Health Care) is not tested for endotoxins at a basic level and at a premium price
  • Unlike Polymorphprep, Ficoll-Paque is not manufactured under EU cGMP compliance.

Alternatives: Polymorphprep™ can be a good substitute for Ficoll-Paque

Een reactie achterlaten Reactie annuleren

Je e-mailadres zal niet getoond worden. Vereiste velden zijn gemarkeerd met *

Recente berichten

  • Plant preservative mixture ppm
  • Plant Preservative Mixture
  • PPM Plant Preservative Mixture – 30 mL
  • PPM
  • Capilia TB- NEO

Recente reacties

    Archieven

    • oktober 2023
    • december 2022
    • november 2021
    • juli 2021
    • mei 2021

    Categorieën

    • antibody purchase
    • Bicistronic Vector
    • biot transfection
    • Blog
    • cd22 antibodies
    • cd8 antibody
    • chromogranin a antibody
    • Cloning Service
    • crispr cas9 antibody
    • cytotoxicity assay kit
    • gfp western blot
    • hmgb1 elisa kit
    • il 22 antibody
    • ppm
    • Product
    • triglyceride assay
    • Uncategorized

    Meta

    • Aanmelden
    • Berichten feed
    • Reacties feed
    • WordPress.org
    © 2025 L’Union des Fédérations d’Apiculture de Wallonie et Bruxelles | Powered by Superbs Personal Blog theme